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Endocrine Disrupters in Sewage Influents - Analytical Method Development
Price: £10
Ref: 04/TX/04/9
ISBN: 1-84057-339-2
A performance tested analytical method has been developed to determine the natural oestrogens, oestrone and 17-b-oestradiol and the synthetic oestrogen ethinyl oestradiol in sewage influent. Samples were extracted after filtration to separate the liquid and solid phase. The liquid phase was extracted using solid phase extraction (SPE) disks and the solid phase was extracted with methanol using accelerated solvent extraction (ASE). The extracts were purified using SPE cartridges. Analysis to determine free steroid oestrogens was undertaken using liquid chromatography mass spectrometry (LCMS) operated in the negative ion electrospray mode. Statistical performance testing of the method shows that the procedure can be used for the routine monitoring of free steroids in sewage influent. The statistically derived limits of detection of the method to determine 17-b-oestradiol, ethinyl oestradiol and oestrone in sewage influent were 2.6, 11.4 and 5.2 ng l-1 respectively in the liquid phase and 65, 32 and 98 ng g-1 respectively in the solid phase.
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Research Update on Oestrogenic Substances in the Environment
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Endocrine Disrupters in Sewage Sludge: Analytical Method Development
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